Kosteletzkya virginica (L.) is a newly introduced perennial halophytic plant. Presently, reverse transcription quantitative real-time PCR (qPCR) is regarded as the best choice for analyzing gene expression and its accuracy mainly depends on the reference genes which are used for gene expression normalization. In this study, we employed qPCR to select the most stable reference gene in K. virgini...

Demodex folliculorum It is one of the most types microscopic external parasites that infect humans, which feeds on fat skin, around hair follicles and causes many diseases, including Perioral dermatitis, Demodectic Blepharitis, Pityriasis folliculorum, Rosacea leads to induction allergic inflammatory reactions against its antigens.The current study aimed detect parasite in humans by using PCR t...

Watermelon is one of the major Cucurbitaceae crops and the recent availability of genome sequence greatly facilitates the fundamental researches on it. Quantitative real-time reverse transcriptase PCR (qRT-PCR) is the preferred method for gene expression analyses, and using validated reference genes for normalization is crucial to ensure the accuracy of this method. However, a systematic valida...

BACKGROUND Efficient screening for detection of colorectal cancer (CRC) at earlier stages reduces its mortality. The purpose of this study was to investigate expression of carcinoembryonic antigen (CEA) and human telomerase reverse transcriptase (hTERT) mRNA in peripheral blood of CRC patients and to present strategies for early detection screen test. METHODS Twenty seven patients in non-meta...

This study involved a death which occurred in four Himalayan griffons housed in Beijing zoo, China. Based on pathogen identification and the pathological changes observed, we did characterize the fungi and Hepatitis E virus (HEV) in four dead Himalayan griffons. Pathological changes were severe. Membranous-like material was observed on the surface of the internal organs. Spleen was necrotic. Fo...

Sarcocystosis considerably occurs in a wide host range including animals, reptiles, humans, and birds. This study was conducted to determine the prevalence of Sarcocystis spp. using abattoir inspection, genetic characterization, as well histopathology water buffaloes two provinces, Elbehera Kafrelsheikh, Egypt. Specimens were collected from esophagus, tongue, masseters 400 slaughtered (n= 215) ...