methods genomic dna of cultured s. agalactiae was prepared and loop-mediated isothermal amplification (lamp) primers were designed based on the camp gene in bacteria. the optimum primer set was selected based on the reaction speed and specificity. the reaction result was monitored visually. the sensitivity and specificity of the lamp method were evaluated and compared with polymerase chain reac...

conclusions species identification of trichophyton is crucially important for epidemiological and phylogenetic purposes and for genotype delineation. rca based on its polymorphisms can be used to generate identification barcodes and as an alternative to dna sequencing; it is a very fast, specific, and economical tool for species identification. results of the 61 putative trichophyton clinical i...

DNA direct amplification from biological samples such as saliva and blood has proven to be successful but can more challenging minute trace DNA.

Sensitive detection of miRNA-21 provides remarkable results for the diagnosis early breast cancer. Here, we fabricated a novel electrochemical biosensor ultrasensitive via in situ reduction gold nanoparticles (AuNPs)-decorated Ti 3 C 2 MXene, combined with cascaded signal amplification strategy, that is, strand displacement DNA walker-induced multi-DNA (product DNA) release and cyclic enzymatic...

Background: Amplification of HER2 is seen in 20-30% of breast cancer cases. Measurement of HER2 gene amplification appears to be of vital importance in planning the treatment schedule for patients with breast carcinoma. The aim of our study was to evaluate HER2 amplification status in malignant and benign breast tumors by differential PCR (dPCR). Materials and Methods: The genomic DNA was ex...

Invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. An early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. Recently, the polymerase chain reaction (PCR) has been used successfully in detecting specific DNA of several pathogen. In this study, nested PCR was used to detect DNA specific for A!.pergiflus s...

Random Amplified Polymorphic DNA (RAPD) analysis of Pistacia vera L. was used to distinguishing sex type in progeny plants. Varied genotypes of the pistachio were analyzed by PCR amplification of a ten base primer previously found to be linked to the expression of a 945bp amplification band in female trees and its absence in males. Results revealed that genetic material from cultivar samples am...