introduction: today, investigators pay attention to natural fluids (such as follicular and amniotic fluid) to use them as a medium or a supplement. using human amniotic fluid (haf) in some researches, not only improved in vitro development of preimplantation embryos but also significantly increased percentage of pregnancy. on the contrary, gianaroli et al. have claimed there was no significant ...

introduction: the aim of this study was to investigate the growth and development of mouse two cell embryo on human uterine polarized monolayers. material and methodes: human endometrial tissue was obtained from patients who had undergone total hysterectomy. then, epithelial cells were isolated by collagenase i 0.25% and cultured on either ecm gel coated millipore filter insert (polarized) or p...

Background: Diabetes mellitus is a metabolic condition characterized by elevated blood glucose levels secondary to absolute impairment of insulin secretion. Maternal diabetes has been demonstrated to adversely affect pre implantation embryo development and pregnancy outcomes. Emerging evidence has implicated that these effects are associated with compromised oocyte competence. Several developme...

The aim of this study was to analyze the incidence of stage of embryonic development, embryo quality and the time of transfer on pregnancy rate in embryo transfer program timed. We were worked a total of 23Holsteincowsasembryo donors, super-ovulated with gonadotropin of pregnant mare serum (PMSG) (2500 3000UI).69 cows were used as recipients of Holstein dairy heifers with an age of20 months and...

The aim of this study was to analyze the incidence of stage of embryonic development, embryo quality and the time of transfer on pregnancy rate in embryo transfer program timed. We were worked a total of 23Holsteincowsasembryo donors, super-ovulated with gonadotropin of pregnant mare serum (PMSG) (2500 3000UI).69 cows were used as recipients of Holstein dairy heifers with an age of20 months and...

background: polycystic ovarian syndrome (pcos) is one of the most common endocrine disorders which cause anovulatory infertility and hyperandrogenism in young women. the common feature in pcos women is increased ovarian androgen secretion which can effect on the prevalence of miscarriage rate. objective: the aim of this study was to investigate the effect of pcos patient's serum on in vitro dev...

Background Little is understood about the regulation of gene expression during early goat embryo development. This study investigated the expression profile of 19 genes, known to be critical for early embryo development in mouse and human, at five different stages of goat in vitro embryo development (oocyte, 8-16 cell, morula, day-7 blastocyst, and day 14 blastocyst). MaterialsAndMethods Stage-...

For sexual reproduction of Allium hirtifolium, collected bulbs were planted in the field. In order to develop ovary embryo and seed setting, control self-pollination and natural open pollination took placed using honey bees under cages. Ovary development and seed setting of A. hirtifolium extraordinary were affected by pollination and there was a significant difference between mean effect of se...

Background: Normal growth of oocytes, embryos and also successful implantation depend on environmental factors such as the secretion of cumulus cells and composition of follicular and tubular fluids. Therefore, addition of serum, conditioned medium (CM) to the culture medium and also co-culture with somatic cells improve mammalian embryo development. So, in the respect to the secretion of diffe...