نتایج جستجو برای: gene specific primers

تعداد نتایج: 1989677  

Background: Splicing by overlap extension (SOE) PCR is used to create mutation in the coding sequence of an enzyme in order to study the role of specific residues in protein’s structure and function. Objectives: We introduced a nested-SOE-PCR (N –SOE-PCR) in order to increase the specificity and generating mutations in a gene by SOE-PCR.   Materials and Methods: Genomic DNA from Bacillus thermo...

بیدخوری , غلامرضا, حجتی نجف آبادی , زهره, متولی باشی , مجید,

Background and purpose: Polymerase chain reaction (PÇR) is a rather quick and accurate method employed for gene detection and isolation. Primer designing is an important issue in this technique and plays a critical role in considering both the genome properties and cloning of the isolated genes. Streptomycin antibiotic is produced by Streptomyces griseus using str gene cluster with more than 25...

خیام نکوئی, سید محتبی, بابائیان جلودار, نادعلی , خادمیان, راحله , مردی, محسن , ناخدا, بابک,

There is a lot of information about genes sequence but their functions are still unknown. So, to fill the gap between structure and function of these sequences many reverse genetic researches have been done. Current experiment studying, how to design gene-specific primers, that can determine single nucleotide diversity and its impact on gene function.This research was condacted at International...

Journal: :journal of crop protection 0
khosrow chehri department of biology, faculty of science, razi university, kermanshah, iran. seyyed meqdad hasani department of biology, faculty of science, islamic azad university, yasuj branch, iran.

wild grasses are the most important primary feedstuffs which are susceptible to contamination with toxigenic fungi belonging to aspergillus spp. in order to explore diversity of aspergillus species associated with the inflorescences of gramineous weeds, infected inflorescences were collected from wild grasses in western parts of iran. fifty-six aspergillus isolates were obtained from all diseas...

Journal: :iranian journal of biotechnology 2015
ali asghar karkhane bagher yakhchali ferdous rastgar jazii bijan bambai saeed aminzadeh

background: splicing by overlap extension (soe) pcr is used to create mutation in the coding sequence of an enzyme in order to study the role of specific residues in protein’s structure and function.objectives: we introduced a nested-soe-pcr (n –soe-pcr) in order to increase the specificity and generating mutations in a gene by soe-pcr.  materials and methods: genomic dna from bacillus thermoca...

Journal: :دامپزشکی 0
وحید نعمان بخش تحقیقات دامپزشکی مرکز تحقیقات کشاورزی و منابع طبیعی استان اصفهان، اصفهان، ایران

one hundred and fifty blood samples were prepared from cattle of a region in isfahan. the extracted dna from blood cells were analyzed by a. centrale (amori strain) specific nested-pcr using primers derived from 16s rrna gene. all blood smears were negative for a. centrale like structures. the results showed that 2 of total 150 blood samples (1.33%) were a. centrale (amori strain) positive by s...

ژورنال: ارمغان دانش 2019

Tyrosinemia is a rare autosomal recessive genetic disease caused by fumarylacetoacetate hydrolase deficiency. 40 different mutation have been recognized related to Tyrosinemia that could be found in all extend of the gene with higher frequency from exon 8 to 14. Because of the size of FAH gene it's impossible to Sequence whole length of the gene by one round of sequencing reaction. Aim of this ...

Journal: :applied biotechnology reports 0
morteza hamedi behnaq molecular biology research center, baqiyatallah university of medical sciences, tehran, iran ali karami molecular biology research center, baqiyatallah university of medical sciences, tehran, iran e-mail: [email protected] ali choopani 2. applied biotechnology research center, baqiyatallah university of medical sciences, teh-ran, iran 3. department of biology, payam noor university, tehran, iran.

abrin known as ribosome inactivating protein’s (rip) it is a high cytotoxic plant protein. high lethality and low cost and easy access to plant and seeds lead to this toxin used in crimes and terrorist acts. because obtaining purified toxins requires advanced laboratory equipment and complex procedures, so, it appears that the perpetrators of such crimes use crude extracts. as a result, remaini...

A.K. Sarker, C. Lyzu, D. Islam, D.C. Roy, L.C. Mohanta, M.A.A. Khan, M.M.K. Hossain, S. Akhter,

Background: Species identification in commercially processed food and feed products is one of the important issues. This study was conducted to develop a genetic method for the detection of pig and cattle species in processed food and feed products using newly designed species-specific primers targeting mitochondrial 12S rRNA gene fragments. Methods: Two sets of specific primers were designed ...

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