the human papillomavirus l1 major capsid protein (hpv l1), the basis of the current vaccines, self-assembles into virus-like particles (vlps). herein, we describe the expression and purification of recombinant hpv16 l1 in e. coli system. the l1 protein was generated in a fused form using an inducible expression system. the recombinant gst-l1 fusion protein migrated as a 82 kda protein in sds-pa...

  Objective(s): To investigate the expression of human papilloma virus (HPV) L1 capsid protein, and human telomerase RNA component (hTERC) in cervical cancer and the role of detection of both genes in screening of cervical cancer.   Materials and Methods: A total of 309 patients were recruited and cervical exfoliated cells were collected. Immunocytochemistry was employed to detect HPV L1 capsid...

objective(s): to investigate the expression of human papilloma virus (hpv) l1 capsid protein, and human telomerase rna component (hterc) in cervical cancer and the role of detection of both genes in screening of cervical cancer.   materials and methods: a total of 309 patients were recruited and cervical exfoliated cells were collected. immunocytochemistry was employed to detect hpv l1 capsid p...

  Objective(s):  Infection by high-risk papillomavirus is regarded as the major risk factor in the development of cervical cancer. Recombinant DNA technology allows expression of the L1 major capsid protein of HPV in different expression systems, which has intrinsic capacity to self-assemble into viral-like particles (VLP). VLPS are non-infectious, highly immunogenic and can elicit neutralizing...

conclusions the results of this meta-analysis showed that gardasil and cervarix vaccines are highly effective against cervical cancer. according to the point that approximately 50% of cervical cancers and human carcinogenicity are related to hpv-16 infection, the bivalent hpv vaccine might have protective effects against hpv-16 cin2-3 lesion and cervical cancer. results by combining the results...

objective(s):  infection by high-risk papillomavirus is regarded as the major risk factor in the development of cervical cancer. recombinant dna technology allows expression of the l1 major capsid protein of hpv in different expression systems, which has intrinsic capacity to self-assemble into viral-like particles (vlp). vlps are non-infectious, highly immunogenic and can elicit neutralizing a...

Background: L1 retrotransposons are the most active mobile DNA elements in human genome. Unregulated L1 retrotransposition may have deleterious effect by disrupting vital genes and inducing genomic instabilities. Therefore, human cells control L1 elements by silencing their activities through epigenetic mechanisms. It has been shown that cell division and heavy metals stimulate the frequency of...

background: l1 retrotransposons are the most active mobile dna elements in human genome. unregulated l1 retrotransposition may have deleterious effect by disrupting vital genes and inducing genomic instabilities. therefore, human cells control l1 elements by silencing their activities through epigenetic mechanisms. it has been shown that cell division and heavy metals stimulate the frequency of...