an experiment was conducted to study the micropropagation of satureja khuzistanica. the seeds were planted in 1/2ms medium. after germination and growth of plantlets, they were subcultured in fresh medium. then, they cultured into medium supplemented by different concentrations of ba and kinetin. results showed that, the highest proliferation (2.4 shoots) was observed at concentrations of 0.5 m...

Apple is one of the most important temperate-zone fruit trees in the world. Micropropagation of apple scion and rootstocks is an important method of rapid production of healthy and disease-free plants. In this research, in vitro propagation of three apple roottocks including Azayesh-Esfahan, Morabbaee-Mashhad and M9 was investigated. Shoot proliferation carried out in two basal media (MS and WP...

Micropropagation of Kainth using nodal explants was carried out in tissue culture laboratory, Department of Fruit Science, Punjab Agricultural University, Ludhiana during 2011-13. The effect of various media {1/2 MS (M1), MS (M2) and WPM (M3)} and growth regulators (BAP, IBA and NAA) on establishment, proliferation and rooting was studied. Per cent necrotic culture was found to be influenced by...

a methodology to regenerate whole plants of fig tree (ficus carica cv. jaami-e-kan) from apical meristem of greenhouse plantlets was developed. two meristematic explants size (0.2-0.4 and 0.5-0.7 mm), two kinds of culture media and three concentrations of 6-benzyladenine (0.5, 1 and 1.5 mgrl-1) were tested as a completely randomize design (crd) with a simple factorial arrangement for establishm...

An efficient and highly reproducible protocol for micropropagation of bird eye chilli Capsicum frutescens was attempted. Murashige and Skoog (MS) medium containing 0.5-3.0 mgl(-1) of 6-benzyladenine (BA), 2-isopentenyl adenine (2iP), kinetin and 0.5-2.0 mg l(-1) of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) along with 1 gl(-1) activated charcoal (AC) were used for shoot regeneratio...

Seeds of Bauhinia acuminata L. were used as an experimental material for micropropagation. The effect of growth regulators concentrations on morphogenetic development was studied. Seeds were germinated on MS medium supplemented with GA3 (0.10, 1.0 and 2.0 mgL -1 ) and without GA3. Satisfactory germination was observed at media with 1.0 mgL -l GA3. Subsequent propagation from plantlet was perfor...

In vitro clonal multiplication of apple rootstock MM 111 using axillary buds and shoot apices were carried out. Vegetative axillary buds of the size of 0.2-2.0 cm and shoot apices measuring 4 mm in length were initiated to shoot proliferation on MS medium supplemented with BA (0.5 - 1.0 mgl(-1)), GA3(0.5 mgl(-1)), with or without IBA(0.05 - 0.1 mgl(-1)). Small size explants showed less phenol e...