So far, recombinant antigens of HIV-1, the etiologic cause of Acquired Immunodeficiency Syndrome (AIDS), have been widely used for the diagnosis and vaccine development. P17 or the matrix protein formed by the proteolytic cleavage of gag is strongly antigenic and is as conserved and immunogenic as p24. In some cases, antibodies to p17 are more prevalent than antibodies to p24 and the decline in...

Staphylococcus aureus carrying PVL gene remain major health problem associated with highly virulent infections. Characterization of such gene is important to know the impact and the functional significance of nucleotide variations. PCR and standard sequencing were performed for twelve Sudanese strains from different sources. Protein structures prediction, modeling and physiochemical analysis we...

Abstract Background Oil flax (linseed, Linum usitatissimum L.) is one of the most important oil crops., However, increases in drought resulting from climate change have dramatically reduces linseed yield and quality, but very little known about how coordinates expression resistance gene response to different level stress (DS) on genome-wide level. Results To explore transcriptional DS repeated ...

MOTIVATION The bottom-up tandem mass spectrometry (MS/MS) is regularly used in proteomics nowadays for identifying proteins from a sequence database. De novo sequencing software is also available for sequencing novel peptides with relatively short sequence lengths. However, automated sequencing of novel proteins from MS/MS remains a challenging problem. RESULTS Very often, although the target...

The yeast two-hybrid (Y2H) system exploits host cell genetics in order to display binary protein-protein interactions (PPIs) via defined and selectable phenotypes. Numerous improvements have been made to this method, adapting the screening principle for diverse applications, including drug discovery and the scale-up for proteome wide interaction screens in human and other organisms. Here we dis...

background: avian infectious bronchitis (ib), with avianinfectious bronchitis virus (ibv) as the causing agent, is a ubiquitous endemicdisease of the chicken with devastating effects on its industry. a viralmembrane surface protein called s  notonly induces neutralizing antibodies but also plays an important role in virusbinding and entry to host cells. technically, s1 protein gene sequencing a...

Mungbean (Vignaradiata L. Wilczek) is an early maturing legume grown predominantly in Asia for its protein-rich seeds. P deficiency can lead to several physiological disorders which ultimately result a low grain yield mungbean. The genetic dissection of PUpE (Puptake efficiency) and PUtE (P utilization traits are essential breeding mungbean varieties with high uptake efficiency. study involves ...

Methodology for determining amino acid sequences of proteins by tandem mass spectrometry is described. The approach involves enzymatic and/or chemical degradation of the protein to a collection of peptides which are then fractionated by high-performance liquid chromatography. Each fraction, containing as many as 10-15 peptides, is then analyzed directly, without further purification, by a combi...

Full-length de novo sequencing from tandem mass (MS/MS) spectra of unknown proteins such as antibodies or proteins from organisms with unsequenced genomes remains a challenging open problem. Conventional algorithms designed to individually sequence each MS/MS spectrum are limited by incomplete peptide fragmentation or low signal to noise ratios and tend to result in short de novo sequences at l...