نتایج جستجو برای: storing method of bacterial dna

تعداد نتایج: 21339251  

ژورنال: Iranian Biomedical Journal 2016
Ilghari, Dariush, Kamyab, Ahmad Reza , Karimi Arzenani, Mohsen, Khamesipour, Ali, Mamnoon, Babak, Naserpour Farivar, Taghi,

Background: Existence of bacterial host cell DNA contamination in biopharmaceutical products is a potential risk factor for patients receiving these drugs. Hence, the quantity of contamination must be controlled under the regulatory standards. Although different methods such as hybridization assays have been employed to determine DNA impurities, these methods are labor, intensive and rather exp...

Journal: :reports of biochemistry and molecular biology 0
fatemeh vahedi tel: +98 511- 8431780; fax: +98 511- 8420430 elnaz ghorbani microbiology department, al-zahra university, tehran, iran tahereh falsafi microbiology department, al-zahra university, tehran, iran

background: dna vaccination with plasmid encoding bacterial, viral, and parasitic immunogens has been shown to be an attractive method to induce efficient immune responses. bacteria of the genus brucella are facultative intracellular pathogens for which new and efficient vaccines are needed. methods: to evaluate the use of a dna immunization strategy for protection against brucellosis, a plasmi...

2011
A. Bandyopadhyay K. Sarkar

The aim of the present study was to develop a simple, rapid and inexpensive protocol of ultrapure bacterial genomic DNA extraction suitable for use in molecular methodology. This communication describes a protocol which uses superparamagnetic bare nanoparticles for isolation and purification of genomic DNA from overnight culture of Escherichia coli XL1 Blue strain. A comparison is also made wit...

Journal: :The Journal of Agriculture of the University of Puerto Rico 1969

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه فردوسی مشهد - دانشکده علوم تربیتی و روانشناسی 1391

the purpose of this study was to examine the status of supervision on departments’ heads at ferdowsi university of mashhad (fum) and realize some applied themes to provide a fit model for supervising departments’ heads in fum. the method was case study under a research category aimed for applied one. using purposive sampling, interviews were conducted with top fum managers, head departments, an...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه علامه طباطبایی - دانشکده اقتصاد 1391

reinsurance is widely recognized as an important instrument in the capital management of an insurance company as well as its risk management tool. this thesis is intended to determine premium rates for different types of reinsurance policies. also, given the fact that the reinsurance coverage of every company depends upon its reserves, so different types of reserves and the method of their calc...

2017
Alessandra Berzuini Marta Spreafico Daniele Prati

Platelet concentrates are universally prepared with a standard method and stored for 5 days at room temperature (20-24°C) in gentle agitation. Currently, there is a renewed interest in the possibility of storing platelet concentrates below the standard temperatures. In fact, cold platelets might be more effective in bleeding patients and have a lower risk of bacterial transmission. Inventories ...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه فردوسی مشهد - دانشکده ادبیات و علوم انسانی دکتر علی شریعتی 1393

this thesis attempts to measure learning styles, self efficacy and intrinsic motivation as predictors of iranian ielts reading comprehension. in order to address this issue, a quantitative study was conducted on some randomly selected intact students at ferdowsi university. these two groups were assigned as they were undergraduate (ba=91) and graduate (ma =74) students; they were all aged betwe...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه تربیت دبیر شهید رجایی - دانشکده ادبیات و زبانهای خارجی 1392

abstract the current study examined the role of emotional intelligence and motivation on language learning of efl learners in iranian context. the participants of this study were 162 female and 100 male junior high school students. these participants were selected by convenient sampling method. to carry out the study two questionnaires were administered to the participants. first, the adap...

2005
E de Madaria R Francés M Pérez-Mateo

Background and aims: Bacterial infections are common complications in patients with acute pancreatitis, and translocation of bacteria from the intestinal lumen is probably the first step in the pathogenesis of these infections. As blood cultures in afebrile patients are usually negative, more sensitive methods to investigate this hypothesis in patients are needed. Our group has recently develop...

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