نتایج جستجو برای: 5utr were generated in 293t cells using third
تعداد نتایج: 17827559 فیلتر نتایج به سال:
Agonist-sensitive intracellular Ca2+ stores may be heterogeneous and exhibit distinct functional features. We have studied the properties of intracellular Ca2+ stores using targeted aequorins for selective measurements in different subcellular compartments. Both, HEK-293T [HEK (human embryonic kidney)-293 cells expressing the large T-antigen of SV40 (simian virus 40)] and HeLa cells accumulated...
OBJECTIVE To construct a lentiviral vector expressing HIV-1 Tat and identify its expression in 293T cells. METHODS The gene fragment of HIV-1 Tat101 was subcloned to lentiviral transfer vector pHAGE-CMV-MCS-IZsGreen, which was named pHAGE-Tat. Then the constructed pHAGE-Tat was used to co-transfect the packing 293T cells, together with the packaging plasmids pMD2.G and psPAX2. The packaged vi...
Background: Human FIX (hFIX) gene transfer into hepatocytes has provided a novel approach for treatment of hemophilia B. To obtain an improved expression of hFIX, the functional hFIX-expressing plasmids with appropriate intron-derived fragments which facilitate transcription and promote an efficient 3′-end formation of mRNAs are required.Objectives: We ai...
Background and purpose: Human pluripotent stem cells (hPSCs) with the ability to differentiate into adult cells have provided a new perspective for treatment of some diseases. But, the efficiency of differentiation methods to generate hematopoietic progenitor cells (HPCs) is faced with multiple challenges. In the present study, we investigated the formation of hemato-endothelial-like structure...
Background and Objective: One of the approaches for treatment of demylination diseases is cell therapy. Stem cells have been used as a source for generating oligodendrocyte. In a previous communication, the oligodendrocyte-like cells (OLCs) were generated from bone marrow stromal cells (BMSCs) using preinducers (dimethyl sulfoxide and retinoic acid) and induction protocol (PDGF, bFGF, heregulin...
with the increasing population and the need for more food, as well as with the development of science and technology, human approach to unnatural and often chemical inputs to increase agricultural production has been a great expansion and problems such as increased cancers, chronic diseases has created environmental pollution. implementation of organic organic is a solution to these problems . ...
هدف این پایان نامه تعیین تعداد مولدهای مینیمال ایده آلها در یک حلقه (جابجایی، یکدار، موضعی و نوتری) است، هر چند تعیین دقیق تعداد مولدها در حالتهایی خاص امکان پذیر است اما در حالتهای کلی روش های موجود بدست آوردن کرانهای مختلف برای تعداد مولدها است. مرجع اصلی این پایانامه [1] می باشد، به علاوه از نتایج جدیدی که در مرجع [2] نیز آمده استفاده کرده ایم. [1]. j. sally. numbers of generator...
CSL is the primary target of the Notch signaling pathway in mammalian cells. It is a DNA binding protein that generally represses transcription in the absence of Notch signaling and activates transcription upon formation of a ternary complex with NICD, the protease-generated intracellular domain of NOTCH: Previous mapping experiments identified the central third of CSL as both necessary and suf...
تاثیر آموزش تفکر انتقادی به شیوه مباحثه بر روی مهارت درک متن یادگیرندگان انگلیسی به عنوان زبان خارجی
the purpose of the present study was to investigate the effect of instruction through debate on male and female efl learners’ reading comprehension, to examine the differences between the performance of male and female participants on the five dimensions of cctst including analysis, evaluation, inference, deductive reasoning, and inductive reasoning, and to examine the differences between male ...
short story as an important literary genre in the recent decade is focused by many of young writers and literary critical with searching structure, content , artistic techniques and cognitive phenomenon of these works , we can show quantity of using some parts of storied literature from new literal techniques of the word and also we can show the usage of native, cultural and social foundation...
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