Aim: The objective of this study was conducted to determine the seroprevalence and S7 gene characterization of bluetongue virus (BTV) of sheep in the West of Iran, during 2007-2008. Materials and Methods: A total 372 sheep blood samples were collected from known seropositive regions in the West of Iran. Anti-BTV antibodies were detected in the serum samples by group specific, C-ELISA. Extractio...

Hand, foot, and mouth disease (HFMD) is an infectious disease caused by human enterovirus 71 (EV71), coxsackievirus A16 (CVA16) and other enteroviruses. It is of interest that other enteroviruses associated with HFMD in Jinan have been rarely reported. The aim of the present study is to detect and characterize the circulating serotypes of non-EV71 and non-CVA16 enteroviruses associated with HFM...

Nested reverse transcription (RT)-PCR for prostate-specific antigen (PSA) and prostate-specific membrane antigen (PSM) can detect circulat ing prostatic cells in patients with prostate cancer. We evaluated the role of a combined screening approach for PSA and PSM in prostate cancer staging. We examined the peripheral blood samples from 136 patients with adenocarcinoma of the prostate (PCA), 15 ...

Dengue virus (DNV) is the cause of dengue infections and Pakistan along with the world is now being overloaded with their patients. The molecular epidemiological studies reveal that there are four serotypes of the DNV circulating throughout the endemic countries. Non-structural glycoprotein (NS-1) causes the damage of hepatocytes along with the activation of the complement system which in turn ...

distribution of infectious hematopoietic necrosis (ihn) was studied in five major provinces producing rainbow trout(oncorhynchus mykiss)fry in iran by indirect fluorescence antibody ifatand nested-rt-pcr techniques. also the effect of time duration was examined on some positive samples after 8 months post sampling. samples of kidney, liver and spleen of rainbow trout fries collected from 27 tro...

soybean mosaic virus (smv) which belongs to the virus family potyviridae, causes a disease in soybean that is present in soybean-growing areas of the world, and is widely distributed in northern iran. detection of smv is very important for disease management. in the present study several serological and molecular (nucleic acid- based) methods of rapid virus detection were compared. serological ...

AIM To determine the frequencies of HGV and TTV infections in blood donors in Hangzhou. METHODS RT-nested PCR for HGV RNA detection and semi-nested PCR for TTV DNA detection in the sera from 203 blood donors, and nucleotide sequence analysis were performed. RESULTS Thirty-two (15.8%) and 30 (14.8%) of the 203 serum samples were positive for HGV RNA and TTV DNA, respectively. And 5 (2.5%) of...

INTRODUCTION AND METHODS Two real time one-step RT-PCR assays were developed for simultaneous detection and typing of influenza A and B viruses and detection of Respiratory Syncytial Virus (RSV). As regard influenza, primers were designed to amplify specific sequences of gene M of A/H1N1, A/H3N2, A/H5N1, A/H7N7 and A/H9N2 viruses and of gene NP of type B viruses belonging both Yamagata and Vict...