How myosin II localizes to the cleavage furrow in Dictyostelium and metazoan cells remains largely unknown despite significant advances in understanding its regulation. We designed a genetic selection using cDNA library suppression of 3xAsp myosin II to identify factors involved in myosin cleavage furrow accumulation. The 3xAsp mutant is deficient in bipolar thick filament assembly, fails to ac...

This paper describes a method for constrained state estimation based on receding horizon optimization. The case here studied corresponds to an optimization horizon of size two and a constraint horizon of size one. It is shown that, in this case, a simple closed-form solution can be obtained. The resulting estimator is called a Rolling Horizon Estimator with Constraint Horizon One. It is shown t...

The eye primordium of the moth, Manduca sexta, shows two different developmental responses to ecdysteroids depending on the concentration to which it is exposed. Tonic exposure to moderate levels of 20-hydroxyecdysone (20E) or its precursor, ecdysone, are required for progression of the morphogenetic furrow across the primordium. Proliferation, cell-type specification and organization of immatu...

In the eggs of the newt, Cynops (Triturus) pyrrhogaster, change in stiffness of the cortex was measured in various regions at the time of the cleavage. Measurements were performed by Mitchison and Swann's cell elastimeter method with a modification, in which two fine pipettes were attached to the surface of one egg at the same time, in order to compare the rigidity of two regions. The stiffness...

Two to 10 /ig/ml cytochalasin B (CB) caused retraction of the first cleavage furrow in Triturus eggs, a spreading of the unpigmented surface from the furrow region and a flattening of the whole egg. CB appears to act against the contractility of the microfilamentous band at mid-cleavage so as to relax the furrow and also to weaken unpigmented surface to allow the egg to flatten. Uncleaved eggs ...

Context Compaction removal and organic amendment application are commonly used to mitigate the compaction-induced declines in crop yield, soil carbon (C) health. However, response of microbial activities nutrient pools combination mill-mud amendments decompaction profile not fully understood. Aims A field trial was conducted at Burdekin, Australia, investigate effects different managements on c...