نتایج جستجو برای: mammalian cell culture

تعداد نتایج: 1924240  

ژورنال: Hormozgan Medical Journal 2013
Abedian Konari, S. , Beiranvand, B. , Beiranvand, E. , Hasannia, H. ,

Introduction: Mammalian cells culture is an important method for producing recombinant proteins, monoclonal antibody, and hormones application. One of the common methods in the monocyte isolation is using flask adherent cell culture in RPMI-1640 medium. We aimed, in this study, cell culture medium using dextrose 5% with autologous plasma to produce inexpensive medium for isolation of monocytes....

Amiri Yekta A Bahrami Nejad E Eyni S, Fatemi N, Gourabi H Sanati MH

Background: Mammalian Cell lines are the main expression system for the production of recombinant therapeutic proteins. Optimization of cell culture condition is performed via alteration in different parameter. Cell culture media plays an important role in cell cycle because of compounds such as amino acids, vitamins, inorganic salts, glucose, and serum as a source of growth factors, trace elem...

Hepatitis B virus (HBV) is a serious global health problem. The development of a safe and effective vaccinewould help infection prevention. Previous hepatitis B vaccine production involved the isolation of the noninfectious particle from chronic HBV carriers. DNA recombinant technology has been used for vaccineproduction without having been contaminated with blood-born infectious ag...

Journal: :Bioprocess technology 1990

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Neural stem cells (NSCs) as a heterogeneous multipotent and self- renewal population are found in different areas in the developing mammalian nervous system, as well as the sub-ventricular zone (SVZ) and the hippocampus of the adult brain. NSCs can give rise to neurons, astrocytes and oligodendrocytes. The aim of this study was to differentiate neural stem cells into noradrenergic–like cells in...

2017
Kristen Bowey-Dellinger Luke Dixon Kristin Ackerman Cynthia Vigueira Yewseok K. Suh Todd Lyda Kelli Sapp Michael Grider Dinene Crater Travis Russell Michael Elias V. McNeil Coffield Verónica A. Segarra

Undergraduate students learn about mammalian cell culture applications in introductory biology courses. However, laboratory modules are rarely designed to provide hands-on experience with mammalian cells or teach cell culture techniques, such as trypsinization and cell counting. Students are more likely to learn about cell culture using bacteria or yeast, as they are typically easier to grow, c...

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