background: repeated in vitro fertilization (ivf) failure together with ovarian hyperstimulation syndrome (ohss) is one of the distressing situations leading couples to search for alternative treatment options. for such patients with polycystic ovarian syndrome (pcos) who have experienced ovarian hyperstimulation syndrome, mild ovarian stimulation with in vitro oocyte maturation could be a prom...

Background: Vitrification of oocytes is a fast-freezing technique, which may affect the quality of the human oocyte, and consequently affects the embryo development, pregnancy and birth. The aim of the current study was to investigate the consequence of in-vitro vitrification on maturation status of immature human oocytes, additionally, expression levels of stress, and apoptosis related genes. ...

Cattle are considered one of the most important pillars of animal production in most countries of the world. As it being a building block in their national security strategies;endeavoring them to increase this animal population by all possible means. During the last three decades;it was clear thatin vitro maturation, fertilization and culturing of bovine oocytes are the most important of these ...

Background: This study examined the effect of including one of two growth factors (100 ng/ml IGF-1 or 20 ng/ ml EGF) in combination with one of two antioxidants (50 μM cysteamine or 50 μM beta-mercaptoethanol) in invitro maturation of buffalo oocytes, followed by fertilization and subsequent development of embryos. Materials and Methods: The oocytes were recovered by in vivo ovum pick-up techni...

Background: Polycystic ovary syndrome (PCOS) is characterized by chronic anovulation and hyperandro genism and affects approximately 5-10% of women of reproductive age. In vitro maturation (IVM) of mammalian oocytes has become an efficient method to produce mature oocytes in order to use in assisted reproductive techniques such as in vitro fertilization (IVF), intra cytoplasmic sperm injection ...

introduction: standard concentrations of antibiotics in culture media are thought to have no detectable toxic effects on the cultured cells. however, since antibiotics are biologically active substances, the possibility that they interfere to some extent with cellular processes occurring in the cultured cells can not always be totally excluded. this study, therefore, was conducted to assess whe...

background: the utilization of immature oocytes derived from stimulated cycles could be of great importance, specially for urgent fertility preservation cases. the aim of the current study was to determine whether in-vitro maturation is more successful before or after the vitrification of these oocytes. materials and methods: the study was performed in a private ivf center. 318 germinal vesicle...

Background: This study was set up to evaluate the effect of ovarian tissue slow freezing on in vitro growth and pattern of maturation genes expression in mouse preantral follicles encapsulated within alginate hydrogel. Materials and Methods: Ovaries of 12-14 days old female NMRI mice were randomly allocated into control and slow freezing groups. In slow freezing group, ovaries were equilibrated...