Ratoon sugarcane plantlets in southern China have suffered a serious chlorosis problem in recent years. To reveal the causes of chlorosis, plant nutrition in chlorotic sugarcane plantlets and the role of manganese (Mn) in this condition were investigated. The study results showed that the pH of soils growing chlorotic plantlets ranged from 3.74 to 4.84. The symptoms of chlorosis were similar to...

Mitigation of ethylene promoted leaf senescence by lysophosphatidylethanolamine (LPE) was studied. Micropropagated ‘Russet Burbank’ potato (Solanum tuberosum L.,) plantlets were grown on MS media in sterile culture tubes. After 2 weeks of growth, tubes were sealed and ethylene gas was applied to obtain 5 nL·L fi nal concentration in the culture tubes. Observations and measurements were taken tw...

DOG(R)1, which encodes 2-deoxyglucose-6-phosphate phosphatase, has been used as a selectable marker gene to produce transgenic plants. In this study, a transformation vector, pBIDOG, which contains the DOG(R)1 gene, was transformed into oil palm embryogenic calli (EC) mediated by Agrobacterium tumefaciens strain LBA4404. Transformed EC were exposed to 400 mg l(-1) 2-deoxyglucose (2-DOG) as the ...

A protocol for rapid multiplication of Adhatoda vasica has been developed through nodal explants from field grown mature plants. The maximum number of shoots, i.e., 7.75 +/- 0.392 differentiated from split nodal halves on MS medium supplemented with BA (10.0 mg/l) during 4 weeks of culture. Maximum number of shoots formed per explant increased to ca. 30 within 6 weeks of subculture on medium co...

As interest in how to increase biomass production through biotechnological means gains traction, focus is turning towards the use of photoautotrophic micropropagation under elevated levels of carbon dioxide (CO2) to maximize plant growth and productivity. The effect of super-elevated CO2 with cold cathode fluorescent lamps (CCFLs) on the photoautotrophic growth of Oncidesa in vitro has been stu...