Encapsulation-dehydration and PVS2-vitrification cryopreservation protocols were evaluated for the long-term conservation of a diverse group of Rubus germplasm. Cold acclimation for a 4-week period prior to cryopreservation was necessary for regrowth of shoot apices from blackberry and raspberry genotypes. For the encapsulation-dehydration protocol, encapsulated apices were pretreated in 0.75 M...

In this study, whether the addition of antifreeze protein (AFP) to a cryopreservative solution (plant vitrification 2 (PVS2)) is more effective in reducing freezing injuries Hosta capitata than PVS2 alone at different cold exposure times (6, 24, and 48 h) investigated. The upregulation C-repeat binding factor 1 (CBF1) dehydrin (DHN1) response low temperature was observed shoots. Shoots treated ...

In vitro shoot tips of Capparis spiunosa were cryopreserved using the vitrification, encapsulation/ dehydration and encapsulation vitrification techniques. In the vitrification procedure, a maximum of 70% regrwoth was obtained when shoot tips were treated with the Plant vitrification solution (PVS2) for 60 min at 0°C and plunged directly into liquid nitrogen. In encapsulation dehydration, shoot...

Ammodendron persicum (Bunge & Boiss.)is a desert shrub specie which grows on some sand dunes and sandy areas of South Khorasan and Sistan-va-Baluchestan provinces in east of Iran. Low distribution and narrow ecological range have put this species under threat. In order to evaluate the possibility of long-term preservation of A. persicum seeds in cryogenic conditions (-196°C), the seeds of speci...

ABSTRACT Aroeira-do-sertão is a Brazilian native species that has been widely explored. Thus, the population of this reduced and techniques for its preservation are essential, such as conservation seeds in liquid nitrogen (LN). The objective work was to evaluate different cryoprotectant solutions cryopreservation aroeira-do-sertão LN (-196 °C). treatments used were: control (7.5±1.5 °C); withou...