sweet lime split bark is a disorder of increasing importance in fars province. previously we showed that a new variant of hop stunt viroid (hsvd-sb) was related to the disorder. it had homology with a noncachexia variant of hop stunt viroid (cvd-iia-117) which causes cracking the bark of trifoliate orange rootstocks. in this study, showed that m.ngobix, bgli and bsri restriction enzymes can cut...

INTRODUCTION Pairs of oligonucleotide primers used in PCR are often designed with restriction sites in their 5' regions. In many cases, the sites are different in the two primers. In this case, amplification generates a target fragment whose termini now carry new restriction sites that can be used for directional cloning into plasmid vectors. The purified fragment and the vector are digested wi...

Restriction analysis of plasmid pHV14 deoxyribonucleic acid isolated from Escherichia coli K-12, Bacillus subtilis, and staphylococcus aureus with restriction endonucleases MboI, Sau3AI, and EcoRII was used to study the methylation of those nucleotide sequences which in E. coli contain the major portions of N6-methyladenine and 5-methylcytosine. The results showed that neither B. subtilis nor S...

interferon is a protein secreted by eucaryotic cells following stimulation by viruses, bacteria, and many other immunogenes. recent medical studies indicate that interferons have effective role in the treatment of virus infections, immunodeficiency and certain types of cancer such as hairy cell leukaemia (hcl). the aim of the present study is to apply yeast strain for secreting human ifnα2b fol...

background: finding shows that there were several problems in the treatment of helicobacter pylori infection such as the emergence of resistance to the antibiotics, the risk of recrudescence, and the high cost of treatment. the ineffectiveness of conventional treatment mechanisms against cancer cells reveals the importance of peptides as a novel therapeutic approach. however, the short length o...

introduction: toxoplasma gondii is a protozoan parasite which is globally prevalent in human and animals. toxoplasma infection is commonly asymptomatic, but can cause serious medical problems in immunocompromised individuals and in fetus. recombinant antigens of the parasite may be helpful in diagnosing the infection more precisely. the goal of this study was to construct and evaluate the funct...