نتایج جستجو برای: sing semi nested pcr methods between june to september of 2012

تعداد نتایج: 22436024  

پایان نامه :دانشگاه آزاد اسلامی - دانشگاه آزاد اسلامی واحد گرمسار - دانشکده علوم انسانی 1392

the purpose of this study was to investigate the effect of task repetition on accuracy of iranian efl learners ’speaking ability. in order to achieve this purpose, a null hypothesis was developed: there is no statistically significant difference between accuracy speaking ability in iranian efl learners by use of task repetition. ; of course i should mention that, beside this null hypothesis, an...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه شیراز - دانشکده علوم انسانی 1391

from 1950s onward, new theories and critical approaches burgeoned across humanities. these theories were context-oriented; as a result, the analysis of discursive practices gained significance. thus, social, political, historical and cultural discourses that have been hitherto marginalized and considered inferior to literary texts, were introduced as important texts to be analyzed by critics. o...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه شهید چمران اهواز - دانشکده ادبیات و علوم انسانی 1390

the aim of this study is to examine the difference between the introverts and extroverts in preferred advice-giving style. furthermore, it explores the difference between l1 (persian) and l2 (english) regarding the giving of advice for the extroverts and introverts separately. it investigates whether an extrovert or introvert gives advice in the same manner in his l1 (persian) and l2 (english) ...

Journal: :Revista da Sociedade Brasileira de Medicina Tropical 2013
Amanda de Faveri Pitz Andrea Cristine Koishi Eliandro Reis Tavares Fábio Goulart de Andrade Eduardo Alexandre Loth Rinaldo Ferreira Gandra Emerson José Venancio

INTRODUCTION Herein, we report a one-tube, semi-nested-polymerase chain reaction (OTsn-PCR) assay for the detection of Paracoccidioides brasiliensis. METHODS We developed the OTsn-PCR assay for the detection of P. brasiliensis in clinical specimens and compared it with other PCR methods. RESULTS The OTsn-PCR assay was positive for all clinical samples, and the detection limit was better or ...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه شاهد - دانشکده هنر 1391

now-a-days graphic design plays a major role in influencing culture, society, business, and customers, in such a way that one could look upon every individual as a potential customer. graphic designers seek to identify customers needs and find an intelligent solution for them. correct identification and understanding of those needs is the first step towards achieving ones goals. marketing knowh...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه اصفهان - دانشکده زبانهای خارجی 1390

the purpose of this study was to investigate iranian efl learners’ beliefs about the role of rote learning (rl) in vocabulary learning strategies; besides, the study examined if english proficiency would influence learners’ vocabulary learning strategy use. this study addresses the need for a clear understanding of the role of rl in efl vocabulary learning by looking at iranian efl learners’ ow...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه مازندران - دانشکده علوم انسانی و اجتماعی 1392

tourism , today as one of the ways to make money, create jobs , and social and political interactions is considered. this paper aims to examine the strengths and weaknesses of tourism development in mahmoud abad ( mazandaran ). the research method in this study is " descriptive - analytical " and to collection of data used field methods such as questionnaires and documentation - library. the sa...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه مازندران - دانشکده علوم انسانی و اجتماعی 1388

the present study set out (a) to examine the categories of pedagogical knowledge related to the act of teaching of novice and experienced teachers as gleaned from their verbal report of what they were thinking about while teaching and (b) to compare the categories of pedagogical knowledge of novice and experienced teachers. the aim of comparing these two groups of teachers was to see whether di...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه امام رضا علیه السلام - دانشکده ادبیات و زبانهای خارجی 1391

translation studies have become an accepted academic subject and books, journals and doctoral dissertations appear faster than one can read them all (bassnet and lefevere, 1990). but this field also brought with itself so many other issues which needed to be investigated more, in the heart of which, issues like ideology, ethics, culture, bilingualism and multilingualism. it is reported that ove...

Journal: :Revista da Sociedade Brasileira de Medicina Tropical 2010
Andrea Cristine Koishi Débora Fonseca Vituri Pedro Sebastião Raimundo Dionízio Filho Alexandre Augusto Sasaki Maria Sueli Soares Felipe Emerson José Venancio

INTRODUCTION Paracoccidioidomycosis is a systemic infection caused by Paracoccidioides brasiliensis. METHODS In this study, a semi-nested PCR for paracoccidioidomycosis diagnosis was developed. The primers ITS1 and ITS4 were used in the first reaction, while the primers MJ03 and ITS1 primer were used in the second reaction. The semi-nested PCR was used to investigate biopsies of five patients...

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